NGS library preparation. Evolved.
your qPCR inhibitions
Researchers at Foundation Medicine have created a test to interrogate base substitutions, indels, copy number alterations and selected fusions across 287 cancer related genes from FFPE tumor samples using massively parallel next generation sequencing.
Researchers at the Broad Institute evaluate four sequencing platforms using human and microbial samples to assess sources of bias in sequencing and sample preparation workflow processes.
Researchers at the Sanger Center investigate many thermostable DNA polymerases along with various reaction conditions for adapter-ligated fragments for Illumina Sequencing in efforts to determine and reduce bias.
KAPA 2G Fast–the use of this second-generation polymerase allowed us to increase our throughput by 50%, while maintaining and even improving the quality of our results. This is really important because it accelerates the process of mutation detection.