Next-Generation PCR

Kapa Biosystems offers a portfolio of best-in-class PCR reagents engineered using our high-throughput molecular evolution platform. The novel DNA polymerases contained within our kits confer dramatic improvements to performance that enable results and applications not possible with wild-type enzymes.

Next-generation PCR is defined as the shift from generalist wild-type enzymes toward designer specialist enzymes. Kapa Biosystems has engineered, high performance reagents for real-time PCR, high fidelity PCR, next-generation DNA sequencing, fast PCR, hot start PCR, crude sample PCR, robust PCR, and molecular diagnostics.

More information on our portfolio of innovative PCR reagents here>>

Gene Expression Analysis

Despite the high precision of qPCR, conclusions drawn from gene expression experiments are often misleading due to differences in amplification efficiency between the gene of interest and the housekeeping gene(s).

KAPA SYBR® FAST qPCR Kits contain an evolved DNA polymerase that exhibits improved speed, processivity and robustness resulting in consistently high amplification efficiencies required for accurate relative quantification, regardless of amplicon length or complexity.

For more detailed information on gene expression and how KAPA SYBR® FAST qPCR Kits can help to improve your results please download our new application notes:

• Gene Expression I – Housekeeping Genes >>
• Gene Expression II – Relative Quantification >>
• For more product information on KAPA SYBR® FAST qPCR Kits >>

Using TaqMan® instead of SYBR Green? Please follow the link to our new KAPA PROBE FAST qPCR Kits >>

Introducing KAPA PROBE FAST qPCR Kits

KAPA PROBE FAST qPCR Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific probe chemistries including TaqMan®, FRET probes, and molecular beacons.

Optimized for versatility and speed – KAPA PROBE FAST qPCR Kits provide fast and reproducible results for genotyping, gene expression analysis, and multiplexing offering:

• Compatibility with all probe-based qPCR applications and instruments
• Fast, reproducible, and precise quantification
• Discrete clusters in SNP genotyping assays
• Multiplex qPCR
• Broad dynamic range
• Highly stable master mix for high-throughput workflows

For more product information >>

Download the product brochure now >>