KAPA HRM FAST

Accurate detection of DNA sequence variations.

High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and EvaGreen® saturating dye optimized for maximum discrimination between sequence variants.

Product Highlights

Maximize differences in sequence variants

  • Engineered to maximize differences in melting behavior
  • Improved sensitivity

Accurately detect Type IV SNPs

  • 95% detection levels, even when competitors fail
  • Reduced reaction times
DNA extracts were prepared from buccal swabs obtained from 36 individuals using KAPA Express Extract DNA Extraction Kits (according to recommended protocols). DNA extracts were used directly in reactions also containing 2.5 mM MgCl2 and 0.2 μM of each primer. PCR and HRM was performed using a fast, 2-step cycling protocol (45 cycles) with 5 second denaturation (95°C) and 30 seconds annealing/extension (60°C).

 Go from cell to SNP quickly

  • Enables extraction, amplification and HRM in less than 2 hours when used in combination with the KAPA Express Extract Kit
Applications
  • SNP genotyping
  • Mutation discovery (gene scanning)
  • Heterozygosity screening
  • DNA fingerprinting
  • DNA methylation analysis
Kit Specifications and Contents / Storage

Kits can be stored for up to 12 months at -20˚C.

Kits include KAPA HRM FAST qPCR Master Mix (2X), which contains KAPA HRM FAST DNA Polymerase, reaction buffer, dNTPs and EvaGreen saturating dye. MgCl2 (25 mM) is supplied separately.

Licensing Information

Certain applications of this product are covered by patents issued to parties other than Kapa Biosystems and applicable in certain countries. Purchase of this product does not include a license to perform any such applications. Users of this product may therefore be required to obtain a patent license depending upon the particular application and country in which the product is used.
 
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056, 6,171,785, and 5,928,907 (claim numbers 12-24, 27-28). The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
 
Kapa Biosystems Inc. is licensed by Biotium Inc. to sell reagents containing EvaGreen dye for use in real-time PCR, for research purposes only.
 
Licensed under U.S. Patent nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.
 
The purchase of this product includes a limited, non-transferable license under specific claims of U.S. Patent Nos. 6,174,670, 6,569,627 and 5,871,908, owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology, Inc. and Roche Diagnostics GmbH, to use only the enclosed amount of product according to the specified protocols. No right is conveyed, expressly, by implication, or by estoppel, to use any instrument or system under any claim of U.S. Patent Nos. 6,174,670, 6,569,627 and 5,871,908, other than for the amount of product contained herein.
 
EvaGreen is a registered trademark of Biotium, Inc.
Rotor-Gene is a trademark of Corbett Research.

 

Specifications

Spec
Description
Starting Material
DNA, cDNA
Input Amount
100 pg - 20 ng

Contents

Component
2X KAPA HRM FAST Master Mix
MgCl2 (25 mM)
Ordering

Kits include KAPA HRM FAST qPCR Master Mix (2X), which contains KAPA HRM FAST DNA Polymerase, reaction buffer, dNTPs and EvaGreen saturating dye. MgCl2 (25 mM) is supplied separately.

Kit Code
Description
Kit Size
List Price
Quantity
KK4201
100 x 20 µL reactions
1 mL
N/A
KK4202
500 x 20 µL reactions
5 mL
N/A
KK4203
1000 x 20 µL reactions
10 mL
N/A
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