Next Generation PCR
Despite the various claims of other reagents suppliers, all commercially available Taq polymerase is the same wild-type enzyme – any differences in performance are due to buffer formulation and/or enzyme concentration.
There are only a limited number of DNA polymerases that have been modified to improve their performance for laboratory applications. Kapa Biosystems’ next-gen PCR reagents are fundamentally different at the protein level; our engineered polymerases contain unique amino acid modifications that confer dramatic improvements to the function of the enzyme.
Our protein engineering capabilities are the result of a high-throughput molecular evolution technology. This platform is being used to develop a portfolio of designer polymerases engineered specifically for applications such as qPCR, Fast PCR, high fidelity PCR, crude sample PCR, next-generation DNA sequencing, and molecular diagnostics.
KAPA2G Fast HotStart DNA Polymerase
KAPA2G Fast HotStart is a second-generation enzyme derived through a process of molecular evolution. KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase.
In addition, KAPA2G Fast HotStart has been shown to achieve higher yields and sensitivity than competitor enzymes across a wide range of amplicon types.
KAPA2G Fast HotStart is particularly well suited for high throughput Fast PCR, offering total reaction times 20 - 70% shorter than conventional PCR assays. Read more >>
KAPA2G Fast DNA Polymerase
A second generation DNA polymerase engineered for extreme speed - also available in hot start for superior specificity and high-throughput PCR.
Designed specifically for speed and high performance, KAPA2G Fast PCR Kits offer Fast PCR that is not merely based on the reduction of cycling times, but on the intrinsic ability of the KAPA2G Fast DNA polymerase to synthesize DNA at a much faster rate.
1 second per kilobase extension rate allows for increased productivity and faster time to results. Read more >>
KAPAHiFi DNA Polymerase
A second-generation polymerase engineered for extreme fidelity and robustness.
KAPAHiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends.
The intrinsic high processivity of KAPAHiFi results in significant improvements in yield, speed, target length, and the ability to amplify difficult templates. Read more >>
KAPA2G Robust DNA Polymerase
A second generation DNA polymerase engineered for extreme robustness and difficult templates.
KAPA2G Robust DNA Polymerase is a highly versatile engineered enzyme that was derived from Kapa Biosystems’ molecular evolution platform.
The novel amino acid mutations in KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, difficult samples, as well as improved tolerance to many common PCR inhibitors. Read more >>
KAPA SYBR qPCR Kits
The high performance of KAPA SYBR® qPCR Kits is based on an engineered polymerase and an optimized buffer system. Engineering of the novel KAPA SYBR® DNA Polymerase was achieved using Kapa Biosystems' molecular evolution technology platform. The result is a unique enzyme, specifically designed for qPCR using SYBR® Green I dye chemistry.
KAPA SYBR® DNA Polymerase has been engineered to perform optimally in stringent qPCR reaction conditions, exhibiting dramatic improvements in signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity. Read more >>
Standard PCR
KAPALongRange DNA Polymerase
The KAPALongRange PCR system is a blend of Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR.
KAPALongRange possesses higher fidelity than single enzyme Taq polymerase. Read more >>
KAPATaq Hot Start DNA Polymerase
KAPATaq Hot Start is a chemically modified version of the single-subunit Taq DNA polymerase enzyme.
Hot start modification eliminates undesirable amplification products resulting from non-specific primer annealing and enzyme activity at room temperature. Many instances of high background amplification and low yields of specific PCR products can be successfully addressed by using KAPATaq Hot Start. Read more >>
KAPATaq ReadyMix
KAPATaq DNA Polymerase is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermusaquaticus, purified from recombinant Escherichia coli.
KAPATaq 2x ReadyMix contains all the components required for PCR except primers and template - simply use PCR-grade water to make up the required reaction volume. The ReadyMix format includes KAPATaq DNA polymerase enzyme and KAPA dNTPs. Read more >>
KAPATaq DNA Polymerase
KAPATaq is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermusaquaticus, purified from recombinant Escherichia coli.
KAPATaq DNA Polymerase is also available with loading dye reaction buffer. The green loading dye will not inhibit the efficiency of the PCR reaction allowing you to save time and effort without compromising performance. Read more >>
KAPA dNTPs
KAPA dNTPs are research grade nucleotides available individually or pre-mixed. Read more >>
KAPA T4 DNA Ligase
KAPA T4 DNA ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
KAPA T4 DNA Ligase is ideally suited for: routine subcloning, recircularization of linear DNA, library construction, linker ligation. Read more >>
KAPA Rapid Ligation System
The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designed to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature.
The KAPA Rapid Ligation System is ideally suited for: routine subcloning, recircularization of linear DNA, library construction, linker ligation. Read more >>