Next Generation PCR

Despite the various claims of other reagents suppliers, all commercially available Taq polymerase is the same wild-type enzyme – any differences in performance are due to buffer formulation and/or enzyme concentration.

There are only a limited number of DNA polymerases that have been modified to improve their performance for laboratory applications. Kapa Biosystems’ next-gen PCR reagents are fundamentally different at the protein level; our engineered polymerases contain unique amino acid modifications that confer dramatic improvements to the function of the enzyme.

Our protein engineering capabilities are the result of a high-throughput molecular evolution technology. This platform is being used to develop a portfolio of designer polymerases engineered specifically for applications such as qPCR, Fast PCR, high fidelity PCR, crude sample PCR, next-generation DNA sequencing, and molecular diagnostics.

For more information on the recommended applications and features of our engineered products please download the Enzyme Selection Guide >>

Technical support form for Standard PCR>>
Technical support form for KAPA SYBR® FAST qPCR Kits>>

KAPAHiFi HotStart DNA Polymerase

A second-generation polymerase engineered for extreme fidelity and robustness. KAPAHiFi HotStart DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. Read more >>
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KAPA Blood PCR Kits

Eliminate the need for DNA purification with the first polymerase engineered for PCR direct from blood. KAPA Blood PCR Kits are available in two optimized, easy-to-use 2x formats containing all PCR components except primers and template (whole blood). Read more >>
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KAPA SYBR FAST qPCR Kits

KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. The kit is based on a novel DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry. Read more >>
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KAPA2G Robust HotStart

KAPA2G Robust HotStart is a highly robust and versatile second-generation enzyme derived through a process of molecular evolution. The novel amino acid mutations in the flagship KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance. Read more >>
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KAPAHiFi DNA Polymerase

A second-generation polymerase engineered for extreme fidelity and robustness. KAPAHiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. Read more >>
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KAPA2G Fast HotStart DNA Polymerase

KAPA2G Fast HotStart is a second-generation enzyme derived through a process of molecular evolution. KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. Read more >>
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KAPA2G Robust DNA Polymerase

A second generation DNA polymerase engineered for extreme robustness and difficult templates. KAPA2G Robust DNA Polymerase is a highly versatile engineered enzyme that was derived from Kapa Biosystems’ molecular evolution platform. Read more >>
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KAPA2G Fast DNA Polymerase

A second generation DNA polymerase engineered for extreme speed - also available in hot start for superior specificity and high-throughput PCR. Designed specifically for speed and high performance. Read more >>
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Standard PCR

KAPALongRange DNA Polymerase

The KAPALongRange PCR system is a blend of Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR. Read more >>
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KAPATaq HotStart DNA Polymerase

KAPATaq HotStart is a antibody-modified version of the single-subunit Taq DNA polymerase enzyme. Hot start modification eliminates undesirable amplification products resulting from non-specific primer annealing and enzyme activity at room temperature. Read more >>
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KAPATaq DNA Polymerase

KAPATaq is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermusaquaticus, purified from recombinant Escherichia coli. Read more >>
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KAPATaq ReadyMix

KAPATaq DNA Polymerase is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermusaquaticus, purified from recombinant Escherichia coli. Read more >>
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KAPA dNTPs

KAPATaq DNA Polymerase is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermusaquaticus, purified from recombinant Escherichia coli. Read more >>
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KAPA T4 DNA Ligase

KAPA T4 DNA ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Read more >>
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KAPA Rapid Ligation System

The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designed to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature. Read more >>
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