KAPA Taq PCR Kits

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KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.

KAPA Taq and KAPA Taq HotStart DNA Polymerase is ideally suited for:

Code Description Kit Contents Qty Unit Prices
KAPA Taq DNA Polymerase
BK1000 KAPA Taq DNA Polymerase (2,500 U) 10 x 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
BK1001 KAPA Taq DNA Polymerase with dNTPs (2,500 U) 10 x 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
BK1002 KAPA Taq DNA Polymerase (5,000 U) 10 x 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
BK1003 KAPA Taq DNA Polymerase with dNTPs (5,000 U) 10 x 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM). *login for pricing
BK1004 KAPA Taq DNA Polymerase with dye (2,500 U) 10 x 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
BK1005 KAPA Taq DNA Polymerase with dNTPs and dye (2,500 U) 10 x 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM). *login for pricing
BK1006 KAPA Taq DNA Polymerase with dye (5,000 U) 10 x 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
BK1007 KAPA Taq DNA Polymerase with dNTPs and dye (5,000 U) 10 x 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM). *login for pricing
KK1006 KAPA Taq ReadyMix (250 x 50 µL rxns) KAPA Taq DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM. *login for pricing
KK1008 KAPA Taq DNA Polymerase with dNTPs (250 U) 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM). *login for pricing
KK1014 KAPA Taq DNA Polymerase (250 U) 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
KK1015 KAPA Taq DNA Polymerase (500 U) 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), and extra MgCl2 (25 mM). *login for pricing
KK1016 KAPA Taq DNA Polymerase wtih dNTPs (500 U) 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix(10 mM) and extra MgCl2 (25 mM). *login for pricing
KK1020 KAPA Taq DNA Polymerase with dye (250 U) 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
KK1021 KAPA Taq DNA Polymerase with dNTPs and dye (250 U) 250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM). *login for pricing
KK1022 KAPA Taq DNA Polymerase with dye (500 U) 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). *login for pricing
KK1023 KAPA Taq DNA Polymerase with dNTPs and dye (500 U) 500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM). *login for pricing
KK1024 KAPA Taq ReadyMix with dye (250 x 50 µL rxns) KAPA Taq DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM and loading dye. *login for pricing
KAPA Taq HotStart DNA Polymerase
KK1508 KAPA Taq HotStart DNA Polymerase (250 U) 250 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM). *login for pricing
KK1509 KAPA Taq HotStart DNA Polymerase with dNTPs (250 U) 250 U, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free), dNTP Mix (10 mM) and MgCl2 (25 mM). *login for pricing
KK1510 KAPA Taq HotStart DNA Polymerase (500 U) 500 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM). *login for pricing
KK1511 KAPA Taq HotStart DNA Polymerase with dNTPs (500 U) 500 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq Buffer (Mg2+ free), dNTP Mix (10 mM) and MgCl2 (25 mM). *login for pricing
KK1512 KAPA Taq HotStart DNA Polymerase - without buffers (2,500 U) 2500 units, 5 U/µL. Wild-type Taq with HotStart antibody. No buffers, MgCl2 or dNTPs supplied. *login for pricing
KK1513 KAPA Taq HotStart DNA Polymerase (2,500 U) 2500 units,5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM). *login for pricing


Product Description

KAPA Taq and KAPA Taq HotStart PCR Kits are supplied with novel buffers designed for optimal enzyme activity and performance. The proprietary formulation facilitates specific primer annealing, which translates to higher yields of specific product when compared to traditional Taq buffers.

The 5x buffer KAPA Taq HotStart Buffer is supplied without MgCl2 for optimal flexibility. KAPA Taq HotStart DNA Polymerase may, however, be used in combination with any standard Taq buffer with a pH of 8.3 or higher.

KAPA Taq and KAPA Taq HotStart DNA Polymerase has 5’→3’ polymerase and 5’→3’ exonuclease activities, but no 3’→5’ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. PCR products generated with KAPA Taq  and KAPA Taq HotStart are A-tailed and may be cloned into TA cloning vectors.


KAPA Taq HotStart outperforms the competition

A 500 bp fragment of the CCR5 gene was amplified using 100 ng, 10 ng, 1 ng or 100 pg of human genomic DNA as template. KAPA Taq HotStart exhibits improved sensitivity, specificity, and yield when compared with competitive hotstart products. All reactions were performed using the manufacturer recommended protocols.


KAPA Taq HotStart improves the specificity and sensitivity in PCR

A 270 bp amplicon was amplified from mycoplasma DNA with KAPATaq or KAPATaq HotStart. Sensitivity was tested using a 10x template dilution series starting with 1 ng of DNA. Higher sensitivity and less primer dimers are observed when using KAPA Taq HotStart.
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DocumentTypeDownload
KAPA Taq ReadyMix MSDS Material Safety Data Sheet Download
KAPA Taq MSDS Material Safety Data Sheet Download
KAPA Taq HotStart MSDS Material Safety Data Sheet Download
KAPA Taq Brochure Product Brochure Download
KAPA Taq ReadyMix TDS Technical Data Sheet Download
KAPA Taq TDS Technical Data Sheet Download
KAPA Taq Mg Free Buffers TDS Technical Data Sheet Download
KAPA Taq HotStart TDS Technical Data Sheet Download