KAPATaq ReadyMix

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KAPATaq 2x ReadyMix contains all the components required for PCR except primers and template - simply use PCR-grade water to make up the required reaction volume. The ReadyMix format includes KAPATaq DNA polymerase enzyme and KAPA dNTPs.

KAPATaq DNA Polymerase is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli. Taq polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleoside triphosphates. Taq possesses 5'→3' polymerase activity, as well as double-strand dependent 5'→3' exonuclease activity. The enzyme lacks 3'→5' exonuclease activity and therefore does not possess a proofreading function.

KAPATaq 2x ReadyMix is also available with loading dye reaction buffer. The green loading dye will not inhibit the efficiency of the PCR reaction allowing you to save time and effort without compromising performance. After PCR cycling simply load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye.

Product Applications

KAPATaq ReadyMix is suited to the following applications:

  • Standard PCR
  • DNA labeling
  • DNA sequencing
  • Many applications for which a high-quality thermostable DNA polymerase is required

Figure 1 : PCR reactions were performed on a 700 bp plasmid target using 1 unit of Taq polymerase in standard reaction buffer as recommended by each supplier. Sensivity of the reaction was tested using a 10x template dilution series starting at 1 ng of DNA. As can be seen from the results, KAPATaq exhibits greater sensitivty as compared with competing suppliers.

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