This research provides a quantitative comparison of different commercially available library kits in regards to minimal bias, yield and time efficiency. Each library preparation kit was tested by starting with the same DNA sample and by probing the amount of DNA remaining after each protocol step. The results show that the kits that combine several steps, such as KAPA HyperPlus and KAPA HyperPrep kits, exhibit some yields 4 to 7 times higher than other kits. Read more
Researchers at the Broad Institute evaluate four sequencing platforms using human and microbial samples to assess sources of bias in sequencing and sample preparation workflow processes.
Researchers at the Sanger Center investigate many thermostable DNA polymerases along with various reaction conditions for adapter-ligated fragments for Illumina Sequencing in efforts to determine and reduce bias.
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