Learn more about how leading researchers are using our directed evolution products to evolve science and power their experiments.
For Research Use Only. Not for use in diagnostic procedures.
If you want to have tight control over your assay performance and save time and money, you need to set the standard up front. The KAPA hgDNA Quantification and QC Kit provides us with a way to determine how a sample will perform in an assay and understand how the DNA is going to behave.
Dr. Robert Daber, Technical Director, Clinical Genomics, Penn Medicine’s Center for Personalized Diagnostics | Featured Customer Profile
I would recommend the KAPA hgDNA Quantification and QC Kit to all CAP/CLIA labs and cancer testing centers because it is the most robust solution for screening both quantity and quality of sample material.
Greg J. Tsongalis, Ph.D., Co-director of the Pathology Translational Research Program serving Dartmouth-Hitchcok Norris Cotton Cancer Center and the Geisel School of Medicine at Dartmouth College | Featured Customer Profile
Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs.
The Broad Institute, Cambridge MA
Kapa Biosystems enzymes outperform others we’ve used. In particular, KAPA Long Range HotStart DNA Polymerase has enabled us to reliably amplify long range templates up to 12 kb for use in downstream next-generation DNA resequencing. KAPA Long Range HotStart outperformed three other competitors’ enzymes in our head-to-head comparisons. Kapa enzymes are the cornerstone of our current work and we use KAPA Long Range HotStart, KAPA2G Fast HotStart, and KAPA HiFi because of their exceptional performance and great value.
Keith Choate, M.D., Ph.D. Department of Dermatology, Yale University School of Medicine, New Haven, CT
I work in a yeast genetics lab at Harvard Medical School where we had largely abandoned the use of colony PCR after finding it too unreliable – success was just too variable to consider it a useful technique. This changed after trying a sample of KAPA2G Robust HotStart DNA Polymerase. We found the success rate with this enzyme to be very high, even with difficult templates. Now, 2G Robust has become our primary enzyme, replacing a whole catalog of other polymerases that we used to keep in stock. We have again begun to use colony PCR regularly, and the only enzyme we use for it is KAPA2G Robust HotStart.
Dan Spatt, Research Manager, Winston Lab. Department of Genetics, Harvard Medical School, Boston MA
KAPA 2G Fast–the use of this second-generation polymerase allowed us to increase our throughput by 50%, while maintaining and even improving the quality of our results. This is really important because it accelerates the process of mutation detection.
Dr. Adriana Heguy, Manager of the Geoffrey Beene Translational Oncology Core Facility at Memorial Sloan Kettering Cancer Center
I switched to the KAPA SYBR® FAST qPCR Kit from Kapa Biosystems because I noticed a significant improvement in PCR reaction efficiency … and our results more reliable.
Dr. Philippe Demouging, Department of Molecular Psychology, University of Basel, Switzerland | View Case Study
We work with yeast and heavily rely on an efficient and reliable colony-PCR protocol to genotype our many yeast strains. After struggling with so many Taq enzymes from different brands, we found that KAPA Taq Extra is the only one working reproducibly and efficiently. On top of this, their Taq is very affordable and comes as a mix containing everything including gel loading dye. We just add our yeast, primers and you reaction is ready! Thus, for yeast colony-PCR, KAPA Taq Extra is robust, reliable, affordable, and convenient.
CRBM - CNRS UMR5237, Montpellier, France
Kapa Biosystems’ innovative approach has resulted in game-changing advances in library preparation. We tested HyperPlus in our own hands and have achieved near 100% conversion rate – far beyond what many thought possible.
Senior Researcher at major UK Genome Centre