NGS

NGS Automation Solutions

Greater accessibility to NGS platforms, lower sequencing costs, and a proliferation of both research and clinical sequencing applications continue to fuel the demand for high-throughput NGS sample preparation. Automation of NGS sample preparation processes, such as library construction, quantification and normalization/pooling offers many advantages, including higher sample throughput, less hands-on time, greater reproducibility, and improved process control.

The KAPA DNA and RNA Library Preparation Kits are optimized for automated, high-throughput NGS library construction. In addition to automation-friendly protocols, 96-reaction library preparation kits contain the necessary overages required by automated liquid handling platforms. We also support Kapa qualified protocols for Illumina® DNA library construction for various liquid handling platforms, while continuously developing methods for new products and platforms.

KAPA Library Quantification Kits provide for accurate, reliable, and high-throughput quantification of NGS libraries prior normalization and pooling for multiplexed capture and/or sequencing. The workflow (sample dilutions and qPCR setup) is easy to automate in either 96- or 384-well format. Validated methods are available for selected platforms.

For Research Use Only. Not for use in diagnostic procedures.

Automated KAPA Library Preparation methods offer:

A choice of platforms: Validated methods are available for the major liquid handling platforms used in NGS.
High-quality libraries: Methods are carefully optimized, and validated to produce similar library yields, complexity and sequence coverage, as libraries prepared manually with our chemistry.
Flexibility: Methods are compatible with a wide range of sample types and inputs, support different barcoding strategies, fragment sizes, batch sizes, and offer control over key library construction parameters for different workflows.
Convenience: Minimal user intervention is required for batches of up to 96 samples.

Instrument Vendor Liquid Handling Platform KAPA Kit Sequencing Platform Source Status
PerkinElmer Sciclone NGS and NGSx HTP Library Preparation Kit Illumina Vendor Available
Hyper Prep Preparation Kit Illumina Vendor Available
HyperPlus Library Preparation Kit Illumina Vendor Available
Stranded RNA-Seq Library Preparation Kit Illumina Vendor Available
Stranded mRNA-Seq Library Preparation Kit Illumina Vendor Available
Stranded RNA-Seq Library Preparation Kit with RiboErase Illumina Vendor Available
Library Quantification Kit Illumina,
Ion Torrent,
454, SoLiD
Vendor Available
Zephyr NGS HTP Library Preparation Kit Illumina Vendor Available
HyperPrep Library Preparation Kit Illumina Vendor In Development
NGS Express HTP Library Preparation Kit Illumina Vendor Available
HyperPrep Library Preparation Kit Illumina Vendor Available
Library Quantification Kit Illumina,
Ion Torrent,
454, SOLiD
Vendor Available
Beckman Coulter Biomek FXp and FX dual-pod HTP Library Preparation Kit Illumina Kapa Available
HyperPrep Library Preparation Kit Illumina Kapa Available
HyperPlus Library Preparation Kit Illumina Kapa Available
Stranded RNA-Seq Library Preparation Kit Illumina Kapa Available
Stranded mRNA-Seq Library Preparation Kit Illumina Kapa Available
Stranded RNA-Seq Library Preparation Kit with RiboErase Illumina Kapa Available
Biomek FXp dual-pod and NX Span-8 Library Quantification Kit Illumina,
Ion Torrent,
454, SOLiD
Vendor Available
Agilent Technologies Bravo NGS workstation HTP Library Preparation Kit Illumina Vendor Available
HyperPrep Library Preparation Kit Illumina Vendor Available
HyperPlus Library Preparation Kit Illumina Vendor Available
Stranded RNA-Seq Library Preparation Kit Illumina Vendor In Development
Stranded mRNA-Seq Library Preparation Kit Illumina Vendor In Development
Stranded RNA-Seq Library Preparation Kit with RiboErase Illumina Vendor In Development
Eppendorf epMotion 5075 TMX HTP Library Preparation Kit Illumina Vendor Available
Hyper Prep Library Preparation Kit Illumina Vendor In Development
HyperPlus Library Preparation Kit Illumina Vendor In Development
Hamilton STAR NGS Workstation Hyper Prep Library Preparation Kit Illumina Vendor In Development
HyperPlus Library Preparation Kit Illumina Vendor In Development
Stranded mRNA-Seq Library Preparation Kit Illumina Vendor In Development
Library Quantification Kit Illumina,
Ion Torrent,
454, SOLiD
Vendor In Development
Tecan Freedom Evo NGS Workstation HTP Library Preparation Kit Illumina Vendor In development
Hyper Prep Library Preparation Kit Illumina Vendor In development
Library Quantification Kit Illumina Vendor In development
Wafergen Apollo 324 Library Preparation Kit Illumina Kapa Guidelines for the use of KAPA reagents with existing DNA library prep methods

 

 Supporting Documentation:

Poster: Automation of chromatin immunoprecipitation sequencing (ChIP-seq) using the Caliper NGS SciClone System. Westerling, et al.

Poster Note: An automated, high-throughput library construction protocol with benefits for low-input applications. Appel, et al.

App Note: Automated library construction using KAPA Library Preparation Kits on the Agilent NGS Workstation

App Note: Automation of ChIP-seq library preparation for next generation sequencing on the epMotion 5075 TMX

App Note: KAPA Library Preparation Kits enable the construction of high-quality NGS libraries on the Apollo 324 automated NGS library preparation system

“Customer story”: Sample preparation: the heart of NGS. Tecan Journal 1/2014, pp. 18–19.