ChIP Sequencing

Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing analysis (ChIP Sequencing or ChIP-seq), is a powerful method for identifying binding sites of DNA-associated proteins throughout the genome.  ChIP is an antibody-based technique used to enrich DNA molecules that interact directly with proteins, including transcription factors, replication-related proteins, and histones. Subsequent library preparation and sequencing enables the understanding of how transcription factors and other chromatin-associated proteins regulate gene expression and epigenetic processes.

The amount of enriched DNA available for sequencing analysis is often limited; therefore, the construction of high-quality libraries from low-input samples is critical. Kapa Biosystems offers library preparation kits that provide increased yields of adapter-ligated library molecules and reduced amplification bias, which translates to higher library diversity, lower duplication rates, and more uniform coverage for low-input applications.

For Research Use Only. Not for use in diagnostic procedures.

Are you interested in constructing ChIP-seq libraries from ultra-low DNA inputs?

Read our new application note, “KAPA Hyper Prep: A streamlined solution for the construction of ChIP-seq libraries from picogram amounts of DNA.

Download our App Note

 

Workflow

  • Sample Preparation

    Sample Preparation

  • Chromatin immunoprecipitation

    Chromatin Immunoprecipitation

  • Library Construction

    Library Preparation

  • Library Amplification

    Library Amplification

  • Library Quantification

    Library Quantification

Available Products by Workflow

Library Preparation

DNA Library Preparation

KAPA Hyper Prep Kit (Illumina®)

  • Construct high-quality libraries from low-input, ChIP-enriched DNA in less than 3 hours

KAPA Library Preparation Kit (Illumina)

  • Construct high-quality libraries using an optimized “with bead” protocol to minimize bias, resulting in superior sequencing coverage and uniformity

KAPA Library Preparation Kit (Ion Torrent™)

  • Construct high-quality libraries with increased yields of adapter-ligated library molecules, resulting in superior sequencing coverage and uniformity
Library Amplification

Library Amplification

KAPA Library Amplification Kits

  • Minimize PCR-induced bias of AT- and GC-rich library molecules for improved sequencing coverage
Library Quantification

Library Quantification

KAPA Library Quantification Kit

  • Accurately quantify adapter-ligated molecules prior to sample pooling and cluster generation or template preparation for optimal sequencing results