ChIP Sequencing
Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing analysis (ChIP Sequencing or ChIP-seq), is a powerful method for identifying binding sites of DNA-associated proteins throughout the genome. ChIP is an antibody-based technique used to enrich DNA molecules that interact directly with proteins, including transcription factors, replication-related proteins, and histones. Subsequent library preparation and sequencing enables the understanding of how transcription factors and other chromatin-associated proteins regulate gene expression and epigenetic processes.
The amount of enriched DNA available for sequencing analysis is often limited; therefore, the construction of high-quality libraries from low-input samples is critical. Kapa Biosystems offers library preparation kits that provide increased yields of adapter-ligated library molecules and reduced amplification bias, which translates to higher library diversity, lower duplication rates, and more uniform coverage for low-input applications.
For Research Use Only. Not for use in diagnostic procedures.
Are you interested in constructing ChIP-seq libraries from ultra-low DNA inputs?
Read our new application note, “KAPA Hyper Prep: A streamlined solution for the construction of ChIP-seq libraries from picogram amounts of DNA.”
Workflow
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Sample Preparation
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Chromatin Immunoprecipitation
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Library Preparation
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Library Amplification
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Library Quantification
Available Products by Workflow
DNA Library Preparation
KAPA Hyper Prep Kit (Illumina®)
- Construct high-quality libraries from low-input, ChIP-enriched DNA in less than 3 hours
KAPA Library Preparation Kit (Illumina)
- Construct high-quality libraries using an optimized “with bead” protocol to minimize bias, resulting in superior sequencing coverage and uniformity
KAPA Library Preparation Kit (Ion Torrent™)
- Construct high-quality libraries with increased yields of adapter-ligated library molecules, resulting in superior sequencing coverage and uniformity
Library Amplification
KAPA Library Amplification Kits
- Minimize PCR-induced bias of AT- and GC-rich library molecules for improved sequencing coverage
Library Quantification
KAPA Library Quantification Kit
- Accurately quantify adapter-ligated molecules prior to sample pooling and cluster generation or template preparation for optimal sequencing results