FFPE Sample Sequencing

Millions of human tissue samples have been preserved with formaldehyde and stored at hospitals and research institutes around the world. These formalin-fixed, paraffin-embedded (FFPE) samples have tremendous clinical value; scientists use them routinely to find biomarkers of disease or treatment outcomes that can be used to design prognostic or diagnostic tests. FFPE samples make it possible to conduct large-scale studies across a variety of phenotypes without the significant expense of recruiting patients to build new cohorts.

Working with FFPE samples presents its own challenges, however, as the formalin-fixation process is damaging and can result in degraded, highly fragmented DNA that is often unsuitable for genomic studies. While FFPE samples are particularly important for cancer research and clinical sequencing, traditional NGS library preparation workflows may not be able to generate sufficient material for analysis without a large amount of amplification.

Kapa Biosystems offers FFPE workflow solutions from sample QC through library quantification, including the KAPA Hyper Prep and HyperPlus kits for the construction of high-quality DNA libraries. The high-performance enzymes and optimized reagents used in these streamlined workflows enable improved yields from FFPE and other challenging samples in comparison to other library preparation methods.

For Research Use Only. Not for use in diagnostic procedures.

Workflow

  • FFPE Sample

    Sample QC

  • Library Preparation

    Library Preparation

  • Library Amplification

    Library Amplification

  • Library Quantification

    Library Quantification

Resources

Available Products by Workflow

FFPE Sample

Sample QC

KAPA hgDNA Quantification & QC Kits

  • Reliably quantify and assess quality of low-input or FFPE samples to optimize library construction yields and workflows
Library Preparation

Library Preparation

KAPA Hyper Prep Kits (Illumina®)

  • Construct high-quality libraries from FFPE and challenging samples in less than 3 hours

KAPA HyperPlus Kits (Illumina)

  •  Generate high-quality libraries from challenging samples using a streamlined workflow that includes fragmentation and library preparation in a single tube
Library Amplification

Library Amplification

KAPA Library Amplification Kits

  • Minimize PCR-induced bias of AT- and GC-rich library molecules for improved sequencing coverage
Library Quantification

Library Quantification

KAPA Library Quantification Kits

  • Accurately quantify adapter-ligated molecules prior to sample pooling and cluster generation or template preparation for optimal sequencing results