Whole Genome Sequencing

Whole genome sequencing (WGS) utilizes next-generation sequencing technologies to determine the complete genome of an organism. Genetic variation, including single-nucleotide polymorphisms (SNPs), insertion-deletion events (indels), and copy number variants, can be identified. Several methods for whole-genome sequencing have been developed, including shotgun and de novo sequencing, that require the construction of a library. During library construction, genomic DNA is fragmented and modified through the addition of sequencing platform-specific adapters. In shotgun sequencing, reads generated from these fragments are then aligned to a reference genome. In the absence of a reference genome, overlapping regions of sequence can be used to reconstruct the genome, a method termed de novo sequencing, which requires larger fragment sizes to facilitate accurate assembly. For all WGS methods, accurate representation of the genome must be preserved through the library construction process.

Kapa Biosystems offers a suite of products from sample QC to library quantification that enable the preparation of high-quality, low-biased DNA libraries, critical for whole genome sequencing analysis.

For Research Use Only. Not for use in diagnostic procedures.

Workflow

  • FFPE Sample

    Sample QC

  • Size Selection

    Size Selection (optional)

  • Library Preparation

    Library Preparation

  • Library Amplification

    Library Amplification

  • Library Quantification

    Library Quantification

Available Products by Workflow

FFPE Sample

Sample QC

KAPA hgDNA Quantification & QC Kits

  • Reliably quantify and assess quality of low-input or FFPE samples to optimize library construction yields and workflows
Library Construction

Library Preparation

KAPA Hyper Prep Kits (Illumina®)

  • Construct high-quality libraries from FFPE and challenging samples in less than 3 hours

KAPA Library Preparation Kits (Illumina)

  • Construct high-quality libraries using an optimized “with bead” protocol to minimize bias, resulting in superior sequencing coverage and uniformity

KAPA Library Preparation Kits (Ion Torrent®)

  • Construct high-quality libraries with increased yields of adapter-ligated library molecules, resulting in superior sequencing coverage and uniformity
Library Amplification

Library Amplification

KAPA Library Amplification Kits

  • Minimize PCR-induced bias of AT- and GC-rich library molecules for improved sequencing coverage
Library Quantification

Library Quantification

KAPA Library Quantification Kits

  • Accurately quantify adapter-ligated molecules prior to sample pooling and cluster generation or template preparation for optimal sequencing results