Whole Genome Sequencing
Whole genome sequencing (WGS) utilizes next-generation sequencing technologies to determine the complete genome of an organism. Genetic variation, including single-nucleotide polymorphisms (SNPs), insertion-deletion events (indels), and copy number variants, can be identified. Several methods for whole-genome sequencing have been developed, including shotgun and de novo sequencing, that require the construction of a library. During library construction, genomic DNA is fragmented and modified through the addition of sequencing platform-specific adapters. In shotgun sequencing, reads generated from these fragments are then aligned to a reference genome. In the absence of a reference genome, overlapping regions of sequence can be used to reconstruct the genome, a method termed de novo sequencing, which requires larger fragment sizes to facilitate accurate assembly. For all WGS methods, accurate representation of the genome must be preserved through the library construction process.
Kapa Biosystems offers a suite of products from sample QC to library quantification that enable the preparation of high-quality, low-biased DNA libraries, critical for whole genome sequencing analysis.
For Research Use Only. Not for use in diagnostic procedures.
Available Products by Workflow
- Reliably quantify and assess quality of low-input or FFPE samples to optimize library construction yields and workflows
- Construct high-quality libraries from FFPE and challenging samples in less than 3 hours
- Construct high-quality libraries using an optimized “with bead” protocol to minimize bias, resulting in superior sequencing coverage and uniformity
- Construct high-quality libraries with increased yields of adapter-ligated library molecules, resulting in superior sequencing coverage and uniformity
- Minimize PCR-induced bias of AT- and GC-rich library molecules for improved sequencing coverage
- Accurately quantify adapter-ligated molecules prior to sample pooling and cluster generation or template preparation for optimal sequencing results