Colony PCR

Colony PCR is a common high-throughput technique for screening bacterial and yeast transformants to confirm the presence or absence of cloned-insert DNA. Despite the obvious advantages of screening transformants directly from cultures or plates instead of first isolating DNA, use of the technique remains limited due to the inherent limitations of Taq DNA polymerase in crude-sample PCR applications. Taq is easily inhibited by debris from bacterial or yeast cells and components of culture media. As a result, inconsistent results are often obtained and only short fragments of cloned inserts can be interrogated.

KAPA2G Robust DNA Polymerase was evolved for high performance in chemically complex reaction conditions. This second-generation polymerase offers superior tolerance to a wide range of common PCR inhibitors, which translates into unrivalled performance in colony PCR.

KAPA HiFi DNA Polymerase, an evolved proofreading polymerase offering superior fidelity, robustness, and speed, is recommended for colony PCR applications that require high fidelity.

For Research Use Only. Not for use in diagnostic procedures.

Available Products

PCR

KAPA2G Robust PCR Kits

  • Improved tolerance to a range of common PCR inhibitors
  • High success rate with commonly used E. coli and S. cerevisae strains
PCR

KAPA HiFi HotStart PCR Kits

  • Extreme fidelity – 100X higher than Taq polymerase
  • Robust amplification of long targets up to 10 kb