Direct Blood PCR
PCR-based genetic testing is a well-established tool for inherited diseases, ancestry, and forensics. Current protocols rely on laborious DNA extraction steps, as wild-type DNA polymerases are completely inhibited by less than 0.2% whole human blood. Poor amplification efficiency is likely the result of the inactivation of the DNA polymerase by multiple inhibitors (e.g. hemoglobin, IgG, lactoferrin, proteases, anticoagulants, and salts) and inaccessibility of the template and/or primers in the crude sample.
Kapa Biosystems has evolved the first DNA polymerase designed specifically for the efficient amplification of DNA fragments directly from whole EDTA blood.* Using KAPA Blood PCR Kits, DNA fragments may be amplified directly from reactions containing whole human blood without pretreatment of blood samples or DNA isolation.
*Data on file
For Research Use Only. Not for use in diagnostic procedures.
- Direct amplification from fresh or frozen whole blood, blood collected in EDTA tubes, or on FTA® Elute Cards, “Guthrie cards”, or filter paper
- Eliminates the need for DNA extraction in genetic testing protocols for humans, other mammals, and birds