Genotyping refers to the process of determining genetic variations among individuals in a species. PCR-based genotyping methods have revolutionized genotyping due to significantly improved sensitivity, reduced turnaround times and simplified workflows. A plethora of PCR-based genotyping strategies–employing end-point and real-time/quantitative PCR (qPCR)–have been developed in recent years. For most genotyping laboratories– higher throughput, greater efficiency (success rates, sensitivity), and shorter turnaround times remain important areas of improvement.
Kapa Biosystems offers products with significant advantages for high-throughput genotyping, offering significantly shorter cycling times, higher yields and sensitivity than wild-type Taq, the ability to perform genotyping assays from crude samples, and flexibility in primer design. These products enable robust performance and higher success rates across a wide variety of template types and amplicons, including AT- and GC-rich sequences and regions that are difficult to amplify with wild-type Taq.
For Research Use Only. Not for use in diagnostic procedures.
- Direct qPCR from crude blood, tissue, and plant extracts
- Sample-to-Cq workflows in <1 hour
- Superior tolerance to carry-over inhibitors
- Robust performance across a wide range of GC content and amplicon length
- Higher yields and sensitivity than wild-type Taq
- Reduce cycling times to decrease turnaround times
- Compatible with all probe-based qPCR applications
- Reduced cycling times to decrease turnaround times
- Stable master mixes for high-throughput workflows
Engineered to maximize differences in melting behavior between sequence variants resulting in accurate SNP genotyping with maximum sensitivity and speed
- Robust performance across a wide range of template and amplicon length
- Improved tolerance to PCR inhibitors
- Improved PCR success rates
- Increase throughput and decrease turnaround time
- Outperform crude extraction methods
- Tolerant to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides
- Direct amplification from plant tissue, crude extracts, and purified DNA
- Rapid DNA extraction from a variety of samples
- Increased PCR success rates