Site-directed Mutagenesis

Site-directed mutagenesis is a powerful tool for the study of protein structure-function relationships, gene expression, and gene regulation. Many different strategies have been developed, most commonly employing complementary oligonucleotides to introduce mutations (additions, deletions, or substitutions) at specific sites in a DNA fragment cloned into a plasmid. Site-directed mutagenesis is often inefficient, resulting in low yields of the mutant plasmid, high backgrounds of parent DNA, and spurious mutations.

KAPA HiFi DNA Polymerase, an evolved proofreading polymerase offering superior fidelity, robustness, and speed, is recommended for site-directed mutagenesis applications. KAPA HiFi is capable of amplifying plasmid targets up to 18 kb in size with high fidelity and speed.

For Research Use Only. Not for use in diagnostic procedures.

Available Products

PCR

KAPA HiFi PCR Kits

  • Extreme fidelity – 100X higher than Taq polymerase and 30X higher than enzyme blends
  • Robust amplification of long targets
  • High speed – 15 sec/kb extension time
PCR

KAPA T4 DNA Ligase

  • KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA
PCR

KAPA Rapid Ligation

  • Designed to ligate blunt- or cohesive- end DNA fragments in 5 minutes at room temperature