KAPA Rapid Ligation System

Fast and efficient ligation

The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designated to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature. KAPA Rapid Ligase contains T4 DNA Ligase, which catalyzes the formation of phosphodiester bond between 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

5-minute ligation

  • Joins blunt- and cohesive-end DNA in only 5 minutes
  • Repairs single-stranded nick in duplex DNA, RNA, or DNA/RNA hybrids

Convenient-use format

  • Ligation is performed at room temperature
  • Kit contains all buffers and enzymes needed
Applications
  • Routine subcloning
  • Recircularization of linear DNA
  • Library construction
  • Linker ligation
Kit Specifications and Contents / Storage

Kits can be stored for up to 12 months at -20°C.

Includes KAPA Rapid Ligase (25 U/µL) and KAPA Rapid Ligation Buffer (2X).

Specifications

Spec
Description
Starting Material
Duplex DNA or RNA
Input Amount
1 – 10 ug/mL

Contents

Component
KAPA Rapid DNA Ligase (25 U/µL)
KAPA Rapid Ligation Buffer (2X)