KAPA T4 DNA Ligase

High-quality ligase.

KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme efficiently joins blunt and cohesive end termini, as well as repairs single-stranded nicks in duplex DNA, RNA, and DNA/RNA hybrids.

For Research Use Only. Not for use in diagnostic procedures.

Applications
  • Routine subcloning
  • Recircularization of linear DNA
  • Library construction
  • Linker ligation
Kit Specifications and Contents / Storage

Kits can be stored for up to 18 months at -20˚C.

KAPA T4 DNA Ligation is available in both standard and rapid versions. The standard version includes KAPA T4 DNA Ligase (5 U/µL) and KAPA Ligation Buffer (5X).

Specifications

Spec
Description
Starting Material
Duplex DNA or RNA
Input Amount
1 – 10 µg/mL

Contents

Component
KAPA T4 DNA Ligase (5 U/µL)
KAPA Ligation Buffer (10X)