KAPA Taq PCR Kits

High quality and performance for routine PCR.

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Taq is supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template—simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

High performance

  • Improved sensitivity, specificity, and yields
  • Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product
Applications
  • High throughput PCR
  • Amplification of low copy DNA templates
  • Multiplex PCR
  • Specific amplification of complex templates
  • RT-PCR
Kit Specifications and Contents / Storage

Kits can be stored for up to 18 months at -20˚C.

Kits include KAPA Taq (or KAPA Taq HotStart) DNA Polymerase (5 U/µL), KAPA Taq Buffer, and MgCl2 (25 mM). Kits are available with and without dNTP Mix (10 mM each). The following reaction buffers are available:

– KAPA Taq Buffer A and B (10X, with 1.5 mM MgCl2 at 1X), supplied with KAPA Taq PCR Kits only
– KAPA Taq Buffer with dye (10X, with 1.5 mM MgCl2 at 1X), supplied with KAPA Taq PCR Kits with dye only
– KAPA Taq HotStart Buffer (5X, Mg-free), supplied with KAPA Taq HotStart PCR Kits only

KAPA Taq HotStart DNA Polymerase is also sold separately.

ReadyMix formulations (2X) of KAPA Taq DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with and without dye), dNTPs and MgCl2 (at a final concentration of 1.5 mM).

Specifications

Spec
Description
Starting Material
DNA, Plasmid DNA, cDNA
Input Amount
≤250 ng for genomic DNA ≤25 ng for less complex DNA

Contents

Component
KAPA Taq Standard or HotStart DNA Polymerase 5 U/µL
10X KAPA Taq Buffer A
10X KAPA Taq Buffer B
10X KAPA Buffer with loading dye (optional)
5X KAPA Taq HotStart Buffer (HotStart kits only)
MgCl2 (25 mM)
dNTP Mix (10 mM each; optional)
Ordering

Kits include KAPA Taq (or KAPA Taq HotStart) DNA Polymerase (5 U/µL), KAPA Taq Buffer, and MgCl2 (25 mM). Kits are available with and without dNTP Mix (10 mM each). The following reaction buffers are available:

– KAPA Taq Buffer A and B (10X, with 1.5 mM MgCl2 at 1X), supplied with KAPA Taq PCR Kits only
– KAPA Taq Buffer with dye (10X, with 1.5 mM MgCl2 at 1X), supplied with KAPA Taq PCR Kits with dye only
– KAPA Taq HotStart Buffer (5x, Mg-free), supplied with KAPA Taq HotStart PCR Kits only

KAPA Taq HotStart DNA Polymerase is also sold separately.

ReadyMix formulations (2X) of KAPA Taq DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with and without dye), dNTPs and MgCl2 (at a final concentration of 1.5 mM).

Component

Kit Code
Roche Cat. No
Description
Kit Size
List Price
Quantity
BK1000
07958447001
Standard kit
2500 U
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BK1001
07958455001
With dNTPs
2500 U
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BK1002
07958463001
Standard kit
5000 U
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BK1003
07958471001
With dNTPs
5000 U
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BK1004
07958498001
With dye
2500 U
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BK1005
07958501001
With dye, with dNTPs
2500 U
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BK1006
07958510001
With dye
5000 U
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BK1007
07958528001
With dye, with dNTPs
5000 U
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KK1008
07958552001
With dNTPs
250 U
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KK1014
07958579001
Standard kit
250 U
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KK1015
07958587001
Standard kit
500 U
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KK1016
07958595001
With dNTPs
500 U
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KK1020
07958617001
With dye
250 U
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KK1021
07958625001
With dye, with dNTPs
250 U
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KK1022
07958633001
With dye
500 U
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KK1023
07958641001
With dye, with dNTPs
500 U
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KK1508
07958765001
HotStart PCR Kit
250 U
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KK1509
07958773001
HotStart PCR Kit, with dNTPs
250 U
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KK1510
07958781001
HotStart PCR Kit
500 U
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KK1511
07958790001
HotStart PCR Kit, with dNTPs
500 U
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KK1512
07958803001
HotStart DNA polymerase, enzyme only
2500 U
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KK1513
07958811001
HotStart PCR Kit
2500 U
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KK1514
07958820001
HotStart PCR Kit
100 U
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ReadyMix

Kit Code
Roche Cat. No
Description
Kit Size
List Price
Quantity
KK1006
07958536001
ReadyMix, 250 x 50 µL reactions
6.25 mL
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KK1024
07958650001
ReadyMix with dye, 250 x 50 µL reactions
6.25 mL
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